Direct load Color Prestained Protein Marker 10-180kDa Biochemical Reagent

Direct-load Color Prestained Protein Marker(10-180 kDa)

Product Information for Protein Marker

Product Description of Protein Marker

This product consists of 10 highly purified and prestained proteins ranging in molecular weight from 8 kDa to 180 kDa(8,17,25,37, 43,55,72,100,130,180kD), among them, 72 kDa is orange band, 8 kDa is green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein

electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!

After the prestained protein is combined with the dye, the molecular weight will change in the gel of different concentrations. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein.

Shipping and Storage about Direct-load Color Prestained Protein Marker(10-180 kDa)

The products are shipped with ice pack and can be stored at -20℃ for two years. For regular use, it can be placed at 4°C, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing!

stock solution composition

62.5 mM Tris-H3PO4(pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300

Instructions with Direct-load Color Prestained Protein Marker(10-180 kDa)

1. After the product is thawed at room temperature, mix gently to fully dissolve the precipitate.

2. Then take an appropriate amount of this product into the gel hole. mini-gel: 3-5 μL; Western blotting: 1.5-2.5 μL; when the

thickness of the gel is greater than 1.5 mm, the loading volume can be appropriately increased.

Cautions for Direct-load Color Prestained Protein Marker(10-180 kDa)

1. The product needs to be returned to room temperature before use to fully dissolve the precipitate. Incomplete protein denaturation at low temperature may lead to different degrees of dispersion of electrophoresis bands.

2. In western blot experiments, large proteins (>100 kDa) in the product may require longer transfer times or higher transfer voltages to complete transfer.

3. This product contains SDS, and the protein has been denatured, so it should not be used as a molecular weight reference standard for natural protein molecular electrophoresis.

4. The product will have deviations in protein size under different electrophoresis conditions, but after they are calibrated by non-prestained protein standards in the same buffer system, they can be used for protein determination of similar molecular weights.

5. At low concentration of gel, low molecular weight protein will swim on the dye front.

6. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!

7. For your safety and health, please wear lab coats and disposable gloves for operation.

8. For research use only!

Attached table Under different electrophoresis buffer conditions, each band of this product indicates molecular weight

Figure1. 12% SDS-PAGE electrophoresis results

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