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Product Details:
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| Product Name: | DNA Ligase | 10299ES40: | 40,000 U |
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| 10299ES42: | 400,000 U | 10299ES83: | 1,600,000 U |
| Input DNA Mole (pmol): | Input DNA(ng)/ [0.66 × Input DNA Average Length(bp)] | Application: | For Research Use ONLY! |
| High Light: | Novel T4 DNA Ligase,400 U/UL DNA Ligase,DNA Ligase Biochemical Reagent |
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Novel T4 DNA Ligase is a single enzyme product that can be used for DNA fragments and adapters ligation during NGS library preparation. The product has been verified by high-throughput sequencing and has excellent quality. This enzyme has efficient ligation ability and is compatible with various samples.
Product Information
| Product name | Cat# | Specification |
| Novel T4 DNA Ligase (400 U/µL) | 10298ES40 | 40,000 U |
| 10298ES42 | 400 KU |
Product Description with Novel T4 DNA ligase (400 U/µL)
Novel T4 DNA Ligase is a single enzyme product that can be used for DNA fragments and adapters ligation during NGS library preparation. The product has been verified by high-throughput sequencing and has excellent quality. This enzyme has efficient ligation ability and is compatible with various samples.
Product Components about Novel T4 DNA ligase (400 U/µL)
| Components | 10298ES40 | 10298ES42 | |
| 10298-A | Novel T4 DNA Ligase (400 U/µL) | 100 µL | 1 mL |
| 10298-B | 10 × T4 DNA Ligase Buffer | 250 μL | 2×1250 µL |
Shipping and Storage
The components are shipped with ice packs and can be stored at -20°C for 2 years.
Unit Definition for Novel T4 DNA ligase (400 U/µL)
The amount of enzyme required to catalyze the ligation of more than 50% of the DNA fragments in a 20 μL ligation reaction system is defined as 1 activity unit (U ) when 6 μg of λDNA Hind III catabolite is reacted at 16 °C for 30 min.
Cautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. For research use only!
Instructions of Novel T4 DNA ligase (400 U/µL)
The kit is compatible with the mainstream commercial end repair and dA-tailing modules for adapter ligation. Please refer to the following table to prepare the reaction system, vortex and spin down the contents and then place at 20 °C for 15 min.
Table 1 Adapter Ligation System
| Component | Volume (μL) |
| dA-tailed DNA | 60 |
| 10 × T4 DNA Ligase Buffer | 10 |
| 50% PEG 6000 | 10* |
| DNA Adapter | x |
| Novel T4 DNA Ligase | 1-5 |
| ddH2O | To 100 |
Note: 50% PEG 6000 is not provided in the kit, you need to prepare it yourself.
Welcome to inquiry for this Novel T4 DNA ligase (400 U/µL), thank you.
Contact Person: Ms. Kris Zhang
Tel: 0086-0769-85914911
