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Product Details:
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| Product Name: | QRT-PCR | Storage: | -20°C |
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| Shipping: | Dry Ice (-70 ℃) | 2×TS MiRNA Reaction Mix: | 200 μl |
| RNase-free Water: | 1 Ml | Passive Reference Dye (50×): | 200 μl |
| High Light: | QPCR QRT PCR SuperMixes,Gene Detection QRT PCR SuperMixes,QRT PCR SuperMixes Biochemical Reagent |
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qPCR and qRT-PCR SuperMixes
Highlights about qPCR and qRT-PCR SuperMixes
• Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA.
• One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube.
• High amplification efficiency, specificity and sensitivity ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).
Applications
Multiple copy and low copy gene detection.
Storage for qPCR and qRT-PCR SuperMixes
at -20°C in dark for two years
Shipping with qPCR and qRT-PCR SuperMixes
Dry ice (-70 ℃)
Specificaton of qPCR and qRT-PCR SuperMixes
| Component | AQ202-01 |
|
miRNA RT Enzyme Mix |
20 μl |
| 2×TS miRNA Reaction Mix | 200 μl |
| Universal miRNA qPCR Primer (10 μM) | 200 μl |
| 2×PerfectStartTM Green qPCR SuperMix | 5×1 ml |
| Passive Reference Dye (50×) | 200 μl |
| RNase-free Water | 1 ml |
Zhang S , Zeng Y , Yi X , et al. Selection of suitable reference genes for quantitative RT-PCR normalization in the halophyte Halostachys caspica under salt and drought stress[J]. Scientific Reports, 2016, 6:30363.
Wu H , Li Z , Yang P , et al. PKM2 depletion induces the compensation of glutaminolysis through β-catenin/c-Myc pathway in tumor cells[J]. Cellular Signalling, 2014, 26(11):2397-2405.
The loss of MiR-139-5p promotes colitis-associated tumorigenesis by mediating PI3K/AKT/Wnt signaling[J]. The International Journal of Biochemistry & Cell Biology, 2015, 69:153-161.
Qiao Y , Mao Y , Wang J , et al. Analysis of liver and gill miRNAs of Larimichthys crocea against Cryptocryon irritans challenge[J]. Fish & Shellfish Immunology, 2016, 59:484-491.
Sun Y , Qiu Y , Zhang X , et al. Genome-wide identification of microRNAs associated with taproot development in radish (Raphanus sativus L.)[J]. Gene, 2015, 569(1):118-26.
If you need more info about this qPCR and qRT-PCR SuperMixes, please contact with us online,thank you
Contact Person: Ms. Kris Zhang
Tel: 0086-0769-85914911
