Biochemical Reagent No Rox QPCR Mix 1000 X 20ul Reactions Accuris QMax Green

Accuris qMax Green, No Rox qPCR Mix, 1000 x 20µl reactionsAccuris qMax Green, No Rox qPCR Mix, 1000 x 20µl reactions

Details of  No Rox qPCR Mix, 1000 x 20µl reactionsAccuris qMax Green, No Rox qPCR Mix, 1000 x 20µl reactions

Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes. These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

qMax Green qPCR Mix is ideal for gene expression analysis, genotyping studies and detection of DNA and cDNA. Both a high ROX™ and low ROX formulation are available for compatibility with all popular qPCR instruments.

Features with No Rox qPCR Mix, 1000 x 20µl reactionsAccuris qMax Green, No Rox qPCR Mix, 1000 x 20µl reactions

• Superior sensitivity and fast cycling with exceptional results
• Ideal for low copy number templates
• Early Ct values and detection across a broad dynamic range
• Ready to use 2x mastermix
• Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy

Cautions

About Operation with Accuris qMax Green, No Rox qPCR Mix, 1000 x 20µl reactionsAccuris qMax Green, No Rox qPCR Mix, 1000 x 20µl reactions

1. Please ensure that the product is completely thawed and thoroughly mixed before use, briefly centrifuge to collect to the bottom of the tube, and place on ice for later use.

2. To avoid cross-contamination between samples and aerosol contamination, it is recommended to prepare the reaction system in a super clean bench.

3. Avoid repeated freezing and thawing of the product during use.

4. For your safety and health, please wear lab coats and disposable gloves for operation.

Experimental Method about 1000 x 20µl reactionsAccuris qMax Green, No Rox qPCR Mix, 1000 x 20µl reactions

a) Primer design

1. Primers must be specific.

2. The Tm value of the primers must be 58-60°C, and the Tm difference of each primer group should be controlled within 1-2°C.

3. The length of primers is generally controlled between 15-30 bp.

b) Probe Design

1. The Tm value of TaqMan probes is approximately 10°C higher (68-70°C) than the corresponding primers.

2. Inhibit dimerization between TaqMan probes and primers.

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