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Product Details:
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| RNA Ladder: | 1ul | TE (pH 8.0): | 2.5ul |
|---|---|---|---|
| Formamide: | 5ul | Formaldehyde: | 2ul |
| 1 Mg/ml Ethidium Bromide: | 0.5ul | Storage Temperature: | -80°C |
| High Light: | Low Range SsRNA Ladder,6.0Kb RNA Marker,Formamide RNA Ladder |
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Low Range ssRNA Ladder and RNA marker - formamide RNA Ladder (0.125 - 6.0 kb)
Details of Low Range ssRNA Ladder and RNA marker
This product is an RNA size standard ladder marker consisting of 9 bands prepared by in vitro transcription.
|
Fragment |
base |
|
|
1 |
6,000 |
|
|
2 |
4,000 |
|
|
3 |
3,000 |
|
|
4 |
2,000 |
|
|
5 |
1,500 |
|
|
6 |
1,000 |
|
|
7 |
500 |
|
|
8 |
250 |
|
|
9 |
125 |
| Component | Quantity | Storage | Description |
|---|---|---|---|
| RNA Ladder | 25 μl x 1 | -80°C | RNA concentration: about 1 μg/μl Storage conditions: 10 mmol/l Tris-HCl(pH 8.0), 1 mmol/l EDTA(pH 8.0) |
| RNA Ladder | 1 μl | RNA sample (3 μl) |
|---|---|---|
| TE (pH 8.0) | 2.5 μl | |
| Formamide | 5 μl | RNA sample buffer (9 μl) |
| 10 x MOPS (pH 7.0)*1 | 1.5 μl | |
| Formaldehyde | 2 μl | |
| 1 mg/ml Ethidium Bromide | 0.5 μl |
Heat at 65°C for 5min. After heat treatment, cool rapidly.
Mix with 2.5 μl of RNA loading buffer*2 and electrophorese in formaldehyde denaturing agarose gel.
Related information of Low Range ssRNA Ladder and RNA marker - formamide RNA Ladder (0.125 - 6.0 kb)
If you have any question or need more info about Low Range ssRNA Ladder and RNA marker - formamide RNA Ladder (0.125 - 6.0 kb) , please inquiry with us online,thank you
Contact Person: Ms. Kris Zhang
Tel: 0086-0769-85914911
